Journal: Burns & Trauma

Article Title: Macrophage-derived exosome piR-50971 exacerbates sepsis-induced myocardial injury by inhibiting autophagy through the upregulation of N6-Methyladenosine modification of mTOR

doi: 10.1093/burnst/tkaf045

Figure Lengend Snippet: PIWIL2 is highly expressed in myocardial cells and tissue in mice with SIMI. ( a ) qPCR results indicated that PIWIL2 expression was significantly elevated in the myocardial tissue under septic conditions. ( b – c ) Western blot analysis verified increased PIWIL2 expression in myocardial tissue of rats with SIMI. ( d ) Immunofluorescence assay showed enhanced expression of PIWIL2 in the myocardial tissue of rats with SIMI. ( e ) Immunofluorescence assay showed that incubation with LPS-Exo markedly increased PIWIL2 expression in AC16 cells compared with that in the CTL-Exo incubation. ( * P < 0.05, ** P < 0.01, *** P <0.001, **** P < 0.0001). SIMI sepsis-induced myocardial injury, PIWIL2 piwi-like protein 2, LPS lipopolysaccharide, Exo exosome, qPCR quantitative polymerase chain reaction

Article Snippet: The membranes were then incubated overnight at 4°C with primary antibodies against mTOR, PIWIL2, and WTAP (all from Proteintech, USA), as well as p62 and LC3 I/II (both from Cell Signaling Technology, USA).

Techniques: Expressing, Western Blot, Immunofluorescence, Incubation, Real-time Polymerase Chain Reaction